retinal pigment epithelium cell line arpe 19  (ATCC)

Journal: Oncology Letters

Article Title: Exploring multiple biomarkers and constructing ferroptosis-associated competing endogenous RNA networks as dual targets in retinoblastoma

doi: 10.3892/ol.2026.15582

Figure Lengend Snippet: ceRNA networks, drug-gene interactions and PCR validation. (A) The ceRNA-regulating networks illustrate protein-coding genes (red circles), miRNAs (blue diamonds) and lncRNAs (green hexagons), with black lines indicating interactions among lncRNA, miRNA and mRNA, where ceRNA refers to ceRNAs. (B) Validation through reverse transcription-quantitative PCR analysis confirms that CDKN2A and IDH2 expression levels are significantly higher in Y79 cells compared to ARPE-19 cells. Data were normalized to GAPDH expression using the 2 −ΔΔCq method, with each experiment performed in triplicate. ***P<0.001 and ****P<0.0001. (C) The drug-gene interaction analysis shows the linkage map of two hub genes, CDKN2A and IDH2 , along with their potential target drugs, including bisphenol A (code: C006780), sodium arsenite (code: C017947), docetaxel (code: D000077143) and hexabromocyclododecane (code: C089796). ceRNA, competing endogenous RNAs; miRNA, microRNA; lncRNA, long non-coding RNA; RB, retinoblastoma.

Article Snippet: The human retinal pigment epithelium cell line ARPE-19 and the human RB cell line Y79 were sourced from the American Type Culture Collection.

Techniques: Biomarker Discovery, Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing

Journal: The Journal of Biological Chemistry

Article Title: Age-dependent induction of ER stress in retinal pigment epithelium impairs phagocytosis via ADAM17-dependent MERTK shedding

doi: 10.1016/j.jbc.2026.111397

Figure Lengend Snippet: Pharmacological induction of ER stress attenuates phagocytic activity in cultured RPE cells. A , schematic diagram of the phagocytosis assay using fluorescein isothiocyanate (FITC)- and pHrodo succinimidyl ester (pHrodo)-conjugated photoreceptor outer segments (POS). B , a representative image of engulfed FITC-POS ( green ) and Hoechst 33,342 ( blue ) with plasma membrane staining 6 h after FITC-POS treatment. The plasma membrane ( gray ) was visualized by PlasMem Bright Red. Scale bar = 10 μm. C , a representative image of pHrodo signal ( yellow ), LAMP1 (magenta) at 24 h after pHrodo-POS treatment. Scale bar = 10 μm. D–F , Tunicamycin (Tm)-induced short-term ER stress reduces phagocytic activity in ARPE-19 and human primary RPE (hRPE) cells. D , experimental timeline for the assays shown in ( E ) and ( F ). E , quantification of fluorescence intensity for FITC-POS and pHrodo-POS in ARPE-19. Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. ### p < 0.001 vs. control (Cont) group (Dunnett’s test). F , quantitative data of fluorescence intensity for pHrodo-POS in hRPE cells. Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. ## p < 0.01, ### p < 0.001 vs. Cont group (Dunnett’s test). G , quantification of phagocytized pHrodo-POS after co-treatment with thapsigargin (Tg). Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. # p < 0.05 vs. Cont group (Student's t test). H , cell death rate following Tm or Tg treatment for 6 h. Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. N.S. > 0.05 vs. Cont group (Dunnett’s test). I – K , long-term ER stress reduces phagocytic activity in ARPE -19 and hRPE. I , experimental timelines for assays shown in ( J ) and ( K ). Quantitative data of fluorescence intensity of pHrodo-POS in ARPE-19 ( J ) and hRPE ( K ). Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. Cont group (Dunnett’s test).

Article Snippet: The human-derived RPE cell line, ARPE-19, was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Activity Assay, Cell Culture, Phagocytosis Assay, Clinical Proteomics, Membrane, Staining, Fluorescence, Control

Journal: The Journal of Biological Chemistry

Article Title: Age-dependent induction of ER stress in retinal pigment epithelium impairs phagocytosis via ADAM17-dependent MERTK shedding

doi: 10.1016/j.jbc.2026.111397

Figure Lengend Snippet: Maturation of ADAM17 mediated by Ca 2+ release from inositol - 1,4,5-trisphosphate receptors contributes to MERTK shedding and dysfunction of POS uptake. A , time-dependent change of the mature form of ADAM17 (matADAM17) in ARPE-19 cells after Tm treatment at 10 μg/ml. Data are presented as mean ± SEM (n = 4). Each point represents one independent sample prepared from separate wells. # p < 0.05 vs. Cont group (Welch's t test). B , expression level of matADAM17 in ARPE-19 after Tm treatment at 1 μg/ml for 54 h. Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. ### p < 0.001 vs. Cont group (Welch’s t test). C , localization of ADAM17 after Tm treatment at 10 μg/ml for 3 h. Representative images of ADAM17 ( yellow ), Golgin-97 ( magenta ), and Hoechst 33,342 ( blue ). Scale bar = 10 μm. Quantitative data of fluorescence intensity of ADAM17 colocalized with Golgin-97 after 1 and 3 h after Tm treatment. Data are presented as mean ± SEM (Cont; n = 97 cells, Tm 1 h; n = 103 cells, Tm 3 h; n = 104 cells). ### p < 0.001 vs. Cont group (Dunnett's T3 test). D – F , expression level of matADAM17 after Tm treatment at 10 μg/ml in the presence of decanoyl-Arg-Val-Lys-Arg-chloromethylketone (CMK) (100 μM, D), 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester (BAPTA-AM) (100 μM, E ), or 2-aminoethoxydiphenyl borate (2-APB) (100 μM, F ). Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. ## p < 0.01, ### p < 0.001 vs. Cont group; ∗∗ p < 0.01, ∗∗∗ p < 0.001 vs. Tm-only treated group (Games–Howell test). G , schematic diagram of ER stress-induced ADAM17 maturation and MERTK shedding. H and I , effect of ADAM17 small interfering RNA (siRNA) treatment on Tm-induced MERTK downregulation in ARPE-19. H , representative immunoblots of MERTK (extracellular domain), ADAM17, and β-actin. I , quantitative data for MERTK (extracellular domain). Data are presented as mean ± SEM (n = 6). Each point represents one independent sample prepared from separate wells. ## p < 0.01 vs. control siRNA (siCont) single-treated group; ∗ p < 0.05 vs. siCont and Tm co-treated group (Student's t test). J and K , effect of ADAM17 siRNA on Tm-induced dysfunction of POS uptake in ARPE-19. J , schematic protocol of the POS uptake assay and ( K ) quantitative data of fluorescence intensity of FITC-POS internalized in RPE cells. Data are presented as mean ± SEM (n = 8). Each point represents one independent well. # p < 0.05 vs. siCont single-treated group; †† p < 0.01 vs. siCont and chloroquine co-treated group; ∗ p < 0.05 vs. siCont, chloroquine, and Tm co-treated group (Kruskal–Wallis test followed by post hoc Bonferroni test).

Article Snippet: The human-derived RPE cell line, ARPE-19, was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Expressing, Fluorescence, Small Interfering RNA, Western Blot, Control

Journal: Archives of Gynecology and Obstetrics

Article Title: Possible role of hyperimmunoglobulin in reducing the risk of maternal–fetal transmission of cytomegalovirus in the valacyclovir era: a case series

doi: 10.1007/s00404-026-08432-0

Figure Lengend Snippet: CMV-specific antibody levels and activity in case 3. Anti-CMV IgG (circles) and IgM (squares) levels at different days after the first detection of CMV-DNA are shown in panel A. The serum neutralization titer against the infection of epithelial (ARPE-19, circles) and fibroblast (MRC-5, squares) cells and the antibody-dependent cell-cytotoxicity (ADCC) against the infection of ARPE-19 cells are shown in panels B and C, respectively. Vertical dotted lines represent the days of the HIG administration. Red points illustrate tests performed right after HIG administration

Article Snippet: For the neutralization assay, we used VR1814 for epithelial cells (ARPE-19, ATCC) and AD169 (ATCC, Manassas, VA, USA), a reference laboratory-adapted strain, for fibroblast cells (HELF, isolated in-house).

Techniques: Activity Assay, Neutralization, Infection